Analytical & Biological Mass Spectrometry Facility

The Analytical & Biological Mass Spectrometry Core Facility offers mass measurements of synthetic products, polymers and whole proteins.The Core is located in room 124J in the BIO5 Keating Bioresearch Building on the North Campus. Facility personnel determine the mass of the molecular components of a sample for structural confirmation and/or identification. Services include confirmation or determination of unknowns, Proteomics, and, in the future, Metabolomics and Lipidomics.

The Core is equipped with mass spectrometers that can measure molecular masses with high mass accuracy, including a Bruker 9.4 Tesla FT-ICR, a Thermo LTQ Orbitrap Velos, and a Thermo Q Exactive Plus Orbitrap. The latter two instruments are dedicated to peptide analysis. The Core also has a second Q Exactive Plus Orbitrap that will be dedicated to the analysis of lipids and metabolites.  Mass measurements of whole proteins and polymers can be made using the Core’s Bruker Autoflex Speed MALDI-TOF. Routine analyses (low mass accuracy) of a variety of compounds are made using the Bruker AmaZon SL Ion Trap, the ABI Sciex 4000 Q-Trap and the Shimadzu GC-MS. Additional equipment in the Core includes a UV-HPLC with a fraction collector, an AKTA FPLC for protein separations, and 1D- and 2D-gel electrophoresis equipment.

Services

  • SDS-PAGE, small gel (7 or 11cm)
  • 2D-PAGE, small gel (11cm)
  • Protein MW determination (MALDI-TOF MS or ESI-MS)
  • HPLC-MS method development
  • Orbitrap MS (high resolution MS)
  • Orbitrap LC-MS/MS of a protein(s) digest (digested by user)
  • Orbitrap LC-MS/MS of a protein(s) in solution (includes digestion)
  • Orbitrap LC-MS/MS of a protein in gel (includes digestion)
  • Chromatography protein/peptide purification
  • Molecular interaction measurements by SPR
  • Abundant protein depletion
  • Protein Assay
  • Sample cleanup (zip tip, protein precipitation, SPE)
  • Gel staining
  • FTICR-MS (high resolution MS)
  • Low Resolution MS (Ion trap MS or MALDI-TOF MS)
  • Low Resolution MS separation (GC-MS or LC-Ion Trap or LC-4000 QTRAP)

Key Instruments

  • Thermo LTQ Orbitrap Velos with CID/ETD/HCD and nanoLC
  • Thermo Q Exactive Plus with HCD and nanoLC
  • Thermo Q Exactive Plus with HCD and UHPLC
  • AB Sciex 4000 QTRAP and capLC
  • Bruker 9.4T Apex Qh FTICR
  • Bruker AmaZon Ion Trap and capLC
  • Bruker Autoflex Speed MALDI-TOF
  • Shimadzu GC-MS
  • Biacore T100 Biosensor
  • GE Healthcare AKTA FPLC

Hours and Location

Monday - Friday, 9:00 AM – 5:00 PM (24/7 Access for Trained Users)

Keating Bioresearch Building, Room 124J, 1657 E Helen Street, Tucson, AZ 85721
For more information and to schedule time, please click here
*Samples may be dropped off at the GATC freezer in the entryway to Room 106

 

Contacts

Krishna Parsawar, PhD
Manager
krishnaparsawar@email.arizona.edu

(520) 626-4161
 

Cynthia David, PhD
Assistant Staff Scientist
cdavid@email.arizona.edu

(520) 626-4173

Yelena Feinstein, MS
Research Specialist
feinstei@pharmacy.arizona.edu

(520) 626-4161

Kristen Kallen-Keck, BS
Analytical Research Coordinator
keck@email.arizona.edu

 (520) 626-4161