Core Facilities

The Machining and Welding Center, or MWC, provides prototype machining, designing, fabrication, welding, consultation, instructional support, and community outreach programs.

The MWC introduces innovative applications to research and development sectors including faculty, staff, students, and private industry.

The MWC is dedicated to providing expertise for research instrument fabrication, committed to continuous improvement of innovative processes and delivery of superior quality products on time, manufactured with expert precision.

The machining facility on site offers a variety of specialized services, provided by a staff with a combined total of over 150 years of machining experience. The facility is well equipped with a wide variety of machines. The MWC is also able to bid on large projects, even within the University. Some services offered by the machining facility include precision machining and grinding, prototype instrumentation, mechanical design, instrument repair, and machine shop safety classes. The fabrication facility also offers a wide variety of services.

Since its establishment, the MWC has contributed to numerous projects, including the building of NASA’s James Webb Space Telescope and of the Spacewatch telescope on Kitt Peak.

Contact

Larry Acedo, manager
Email: lacedo@email.arizona.edu

Or visit: Gould-Simpson Building 77, Room 235

Machining & Welding Center overview Core Facilities Fair 2017

The Kuiper Materials Imaging & Characterization Facility provides access to cutting-edge instrumentation for electron microscopy – the backbone of nanotechnology – and Raman spectroscopy. Experienced personnel can assist and facilitate research.

Electron microscopy exceeds the resolution of light microscopy and enables elemental analysis to determine the structure and content of materials for identification. Electron microscopy is the backbone of nanotechnology, with the ability to image the surface of a whole insect down to nanometer particles in an SEM. The TEM with atomic composition resolution is ready for the return of dust from the OSIRIS-REx mission – because every atom tells a story.

The Kuiper Materials Imaging & Characterization Facility regularly works with investigators in the UA’s College of Agriculture and Life Sciences, College of Engineering, and College of Science, as well as industry partners dedicated to the research and development of semiconductors, medical devices, lithium batteries, and mining.

Contact

Email: ImagingKuiper@email.arizona.edu

The Magnetic Resonance Research Facility (MRRF) supports MRI research within the University community and for industry partners in pharmaceuticals, health care, veterinary care, and behavioral sciences.

The MRRF regularly works with researchers in the UA’s College of Science and College of Medicine, among others, as well as industry partners in pharmaceuticals, health care, veterinary care, and behavioral sciences.

The MRRF is able to support work that addresses a wide range of research questions, including questions related to arthritis, traumatic brain injuries, PTSD, cancer treatment efficacy, depression, kidney transplant viability, CPR, Alzheimer’s, complex grief, and healthy aging.

Contact

Scott Squire
Email: imagingMRI@email.arizona.edu
Phone: (520) 245-6272

Schedule user time here

Imaging Cores, Biomedical Research Laboratory – MRI overview - Core Facilities Fair 2017

The Analytical & Biological Mass Spectrometry Core Facility offers mass measurements of synthetic products, polymers and whole proteins.The Core is located in room 124J in the BIO5 Keating Bioresearch Building on the North Campus. Facility personnel determine the mass of the molecular components of a sample for structural confirmation and/or identification. Services include confirmation or determination of unknowns, Proteomics, and, in the future, Metabolomics and Lipidomics.

The Core is equipped with mass spectrometers that can measure molecular masses with high mass accuracy, including a Bruker 9.4 Tesla FT-ICR, a Thermo LTQ Orbitrap Velos, and a Thermo Q Exactive Plus Orbitrap. The latter two instruments are dedicated to peptide analysis. The Core also has a second Q Exactive Plus Orbitrap that will be dedicated to the analysis of lipids and metabolites.  Mass measurements of whole proteins and polymers can be made using the Core’s Bruker Autoflex Speed MALDI-TOF. Routine analyses (low mass accuracy) of a variety of compounds are made using the Bruker AmaZon SL Ion Trap, the ABI Sciex 4000 Q-Trap and the Shimadzu GC-MS. Additional equipment in the Core includes a UV-HPLC with a fraction collector, an AKTA FPLC for protein separations, and 1D- and 2D-gel electrophoresis equipment.

Services

• SDS-PAGE, small gel (7 or 11cm)
• 2D-PAGE, small gel (11cm)
• Protein MW determination (MALDI-TOF MS or ESI-MS)
• HPLC-MS method development
• Orbitrap MS (high resolution MS)
• Orbitrap LC-MS/MS of a protein(s) digest (digested by user)
• Orbitrap LC-MS/MS of a protein(s) in solution (includes digestion)
• Orbitrap LC-MS/MS of a protein in gel (includes digestion)
• Chromatography protein/peptide purification
• Molecular interaction measurements by SPR
• Abundant protein depletion
• Protein Assay
• Sample cleanup (zip tip, protein precipitation, SPE)
• Gel staining
• FTICR-MS (high resolution MS)
• Low Resolution MS (Ion trap MS or MALDI-TOF MS)
• Low Resolution MS separation (GC-MS or LC-Ion Trap or LC-4000 QTRAP)

Key Instruments

• Thermo LTQ Orbitrap Velos with CID/ETD/HCD and nanoLC
• Thermo Q Exactive Plus with HCD and nanoLC
• Thermo Q Exactive Plus with HCD and UHPLC
• AB Sciex 4000 QTRAP and capLC
• Bruker 9.4T Apex Qh FTICR
• Bruker AmaZon Ion Trap and capLC
• Bruker Autoflex Speed MALDI-TOF
• Shimadzu GC-MS
• Biacore T100 Biosensor
• GE Healthcare AKTA FPLC

Hours and Location

Monday - Friday, 9:00 AM – 5:00 PM (24/7 Access for Trained Users)

Keating Bioresearch Building, Room 124J, 1657 E Helen Street, Tucson, AZ 85721 For more information and to schedule time, please click here.  *Samples may be dropped off at the GATC freezer in the entryway to Room 106

 

Contacts

Krishna Parsawar, PhD Manager krishnaparsawar@arizona.edu (520) 626-4161

Lawrence Walker, PhD Assistant Staff Scientist larrywalker@arizona.edu (520) 626-4161

Yelena Feinstein, MS Research Specialist feinstei@pharmacy.arizona.edu (520) 626-4161

Kristen Kallen-Keck, BS Analytical Research Coordinator keck@arizona.edu  (520) 626-4161