Flow Cytometry Shared Resource

The Flow Cytometry Shared Resource (CCF) offers instrumentation to analyze, quantify, and sort cells and particles in a fluid suspension.

Flow cytometry is a powerful tool that can measure functional and structural characteristics of heterogeneous mixtures of cells and particles in suspension. As a shared resource of Arizona Research Laboratories and the UA Cancer Center, the Cytometry Core Facility, or CCF, offers instrumentation to analyze, quantify, and sort cells and particles in a fluid suspension through the use of flow cytometry.

The CCF provides the knowledge and skills necessary to competently perform cell sorting, acquisition, and analysis functions, and interpret the results of those functions. The Facility also offers information about new techniques, instrumentation, and applications of flow cytometry provided through biotechnology-sponsored workshops and seminars.

The cell-sorting function of flow cytometry can physically separate these cells into different classes. Using this technique, the CCF gives researchers the capability to analyze and sort cells by differences in physiology, metabolism, morphology, and other characteristics. The ability to distinguish different cell types is limited only by the ability to attach specific fluorescent dyes to the cells.

  • Flow cytometric analysis (assisted and unassisted usage available)
  • High-speed aseptic cell sorting
  • Consultation in experimental design and data interpretation

Data Reduction Assisted

$52

FACSAria Acquisition

$101

FACSAria Sorting

$123

FACSCanto II Assisted

$45

FACSCanto II Training

$45

FACSSCanto II Unassisted Use

$20

LSR Assisted Acquisition

$73

LSR Training

$73

LSR Unassisted

$32

  • The FACScanto II (BD Biosciences, San Jose, CA) is an analyzer capable of acquiring and analyzing data from six fluorescence parameters and has two lasers: 488nm and 640nm.
  • The FACSAria III (BD Biosciences) is a cell sorter equipped with five lasers: 355nm, 405nm, 488nm, 407nm, and 633nm, and offers 17 fluorescence channels. The sorting function of this instrument enables the researcher to physically separate the individual cells and particles from a mixed population. Viable cells can be recovered for further study or culture.
  • The LSRII (BD Biosciences) is an analyzer capable of acquiring and analyzing data from 16 fluorescence parameters and has five lasers: 355nm, 405nm, 488nm, 532nm, and 640nm.

Contacts

New users are encouraged to meet with the manager of the core service to discuss their needs, and learn how to sign up for access to the scheduler.

Call Mark Curry, manager, at (520)-621-2074, or email markcurry@email.arizona.edu

Flow Cytometry Shared Resource overview - Core Facilities Fair 2017